Our lab is interested in the crosstalk between the cerebrovascular system and the nervous system in Alzheimer’s disease (AD). To identify molecular targets and biomarkers underlying cerebrovascular deficits in AD, we are investigating cerebrovascular dysfunction and blood-brain barrier damage in rodent AD models and postmortem specimens of AD patients using various biochemical & histochemical techniques, in vivo fiberoptic spectroscopy, in vivo two photon imaging, MRI, and 3D Immunostaining combined with iDISCO tissue clearing.

In Vivo Imaging

MRI Analysis of Blood Flow

In Vivo Fiberoptic Spectroscopy

We have developed a new fiber optic method for measuring vascular reactivity in freely moving mice. Current techniques including fMRI and Laser Doppler flowmetry, can measure neuronal activity-mediated cerebral perfusion changes, but both techniques require animals to be anesthetized to minimize movement artifacts, which in turn makes it impossible to analyze cerebral perfusion of freely moving animals during behavioral tasks. In addition, anesthesia is known to modify the coupling relationship between neural and vascular responses. Combining a new fiberoptic method with in vivo electrophysiological recording or chemogenetics, we will analyze the neuronal activity-dependent vascular change in several different brain regions of freely moving AD mice compared to controls and investigate the molecular mechanism how cerebrovascular abnormalities affect cognitive dysfunction in AD.

Analysis of fibrin Clots Using Electron Microscopy

Normal Fibrin Clot

Fibrin Clot with Amyloid Beta

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